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Colon

Physical Characters :

Colour : greenish yellow – Odour : aromatic odour – Taste : slightly bitter.

Chemical Composition :

NO

Component

Result

1

Carbohydraate & Glycosides
Triterpenes & steroids

+++ve

2

sesquiterpene lactones

+++ve

3

Flavenoids

+ve

4

Alkaloids or nitrogenous basic

++ve

5

 compounds

+ve

6

Volatile oils

++ve

7

Coumarine

+ve

8

Tannin

-ve

9

Anthraqunone

-ve

Volatile oil investigation :

Preparation by hydrodistillation : ( E.P. 1984 ) The GLC investigation :

Result :

No

RT

TextFatty acid

% Conc

1

0.683

unknown

0.90

2

4.067

caprylic

0.90

3

4.950

pelargonic

0.026

4

12.833

Myristalic

0.028

5

14.333

palmitoleic acid

15.923

6

15.483

Margaric

3.421

7

18.361

linoleic acid

32.51

Microscopically examination

  • Pollen grain spherical with spherical spiny exinle, has 3 germinal furrows.
  • Fragments of petals with papillosed cells.
  • Fragment of fibrous layer of another which is thick and lignified.
  • Fragment of crystal layers : parenchyma cells contain Ca OX. crystals prisms.
  • Vessels: spiral reticulate, pitted thick, lignified.
  • Elongated cells
  • Fibers group: elongated, fuseforme, thick lignified.

Preiparation of fatty acids

  • N. Hexane ext. was reflexes 2N KOH ( alcoholic solution ) for 4 hours. Extract with ether (3 x 300 ml)
  • Add to the aqueous extract, Hcl > till the PH is acedic. Extract with either (3 x 300 ml)
  • Methylation by using Diazomethane.
  • GLC investip ation of fatty acids.

Result are listed in this table :

No

RT

Fatty acid

% Conc

1

3.022

Carene

0.06

2

3.212

a thujene

0.12

3

3.65

Tricyclene

0.32

4

7.968

a hellandence

18.83

5

8.431

a pinene

0.02

6

9.029

B- pinene

5.57

7

15.826

geraniol

14.61

8

23.527

Elimicin

18.2

9

24.31

Azulene

0.182

Side effects :

There are no side effects

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